Pfizer Biochemist/Enzymologist (non in La Jolla, California
The Biochemistry Department of Pfizer Oncology Research & Development in La Jolla, CA is seeking a full time non-PhD scientist to join our drug discovery team. The enzymologist/biochemist will make important contributions to the discovery and pre-clinical development of oncology therapeutics, and will contribute to our molecular and biochemical understanding of enzymes that mediate epigenetic modifications of histone proteins and DNA.
The scientist will be actively engaged in drug discovery activities for modulators of epigenetic targets, including the characterization of epigenetic enzymes and their inhibitors as well as histone and DNA methylation profiling and selectivity screening.
In particular, the biochemist will be responsible for the development and validation of robust, reproducible plate-based enzyme assays. This will include kinetic and mechanistic studies to characterize enzyme targets, biochemical pathways, and mechanism of action of lead compounds.
Responsible for testing compounds in biochemical assays, data analysis, and proper database publication of data.
Participate on project teams, providing insight and technical expertise required for project progression. The individual will work closely with cell biologists and medicinal chemists, and apply strong biochemistry knowledge/skills to oncology small molecule drug discovery and development of innovative therapies.
Develop and execute kinetic and mechanistic enzyme assays to understand epigenetic protein targets, their function and regulation, along with determining mechanism and binding site location of inhibitors, and protein/ligand SAR.
Responsible for the development and performance of robust, automated biochemical assays for support of a cancer epigenetic drug discovery platform.
Participation on multi-disciplinary project teams, including oral presentation of results, and contribution to the understanding of the biochemical pathways associated with tumor epigenetic changes associated with tumor development and progression is also expected.
BS degree in biological sciences with 6+ years', or MS degree with 5+ years', experience in understanding the enzymes and proteins associated with cancer epigenetics.
Experience in the development of assays and bioanalytical methods for studying enzyme activity and mechanism and for evaluating inhibitor potency and mechanism.
Must have strong technical abilities- be skilled in the biochemical evaluation of enzymes including enzyme kinetics and in the development of automated plate-based biochemical assays.
Strong interpersonal and communication skills required.
Preference given to candidates with pharmaceutical/biotech drug discovery experience in evaluating small-molecule inhibitors against epigenetic enzyme targets such as histone methyltransferase or histone deacetylases
Experience in enzyme kinetics and mathematical modeling strongly preferred.
Experience in Mass Spectrometry
Must be able to work at a lab bench.
Work with standard radioactive materials (3H, 32P, etc).
Requires participation on multi-disciplinary project teams. Frequent interaction with scientists with expertise in biochemistry, chemistry, structural biology and protein purification. Requires the ability to work with external vendors to validate enzyme assays for routine inhibitor evaluation and HTS.
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Eligible for Employee Referral Bonus
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